RESOURCE CENTRE

RESOURCES

We offer material resources of broad interest to the scientific community, including vectors and markers for plant and yeast transformation, as well as specialist seed lines. A small fee will be charged for markers and seeds to cover handling and postage. Contact us or see here for more information.

***Sequence information is provided in genebank (*.gb) files; you can either open them directly with DNA software (VectorNTI, clc workbench, editseq) or simply use a text editor tool.

UBQ10 Vectors

Promoter-driven Gateway vectors for fluorescent tagging of proteins.

For the use of UBQ10 Promoter Driven Vectors please cite:

Grefen,C., Donald,N., Hashimoto,K., Kudla,J., Schumacher,K., and Blatt,M.R. (2010) “A ubiquitin-10 promoter-based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression studies.” Plant J. 64: 355-65

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Yeast Vectors

Vectors and strains for split-ubiquitin screening and assays.

For the use of Gateway SUS Vectors please cite:

Grefen C, Obrdlik P and Harter K (2009) “The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS).” Methods Mol. Biol. 479:217-33.

For the use of SUS Yeast Strains please cite:

Obrdlik, et al. (2004) “K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions.” PNAS 101:12242-7.

Grefen C, Lalonde S and Obrdlik P (2007) “Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins.” Curr. Protoc. Neurosci. Chapter 5: Unit 5.27.

For the use of SUS vector designed for soluble baits please cite:

Karnik, R., Zhang, B., Waghmare, S., Aderhold, C., Grefen, C., and Blatt, M.R. (2015). Binding of SEC11 indicates its role in SNARE recycling after vesicle fusion and identifies two pathways for vesicular traffic to the plasma membrane. Plant Cell 27, 675-694.

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Bicistronic and Multicistronic Vectors

Bicistronic vectors for fluorescence co-expression tagging of proteins in Arabidopsis.

Multicistronic vectors for Secretory Traffic Assay

For the use of the Bicistronic Vector please cite:

Chen,Z.H., Grefen,C., Donald,N., Hills,A., and Blatt,M.R. (2011) A bicistronic, Ubiquitin-10 promoter-based vector cassette for transient transformation and functional analysis of membrane transport. Plant Cell and Environment, 34:554-564.

For the use of the Multicistronic Vectors please cite:

Karnik, R., Grefen, C., Bayne, R., Honsbein, A., Kohler, T., Kioumourtzoglou, D., Williams, M., Bryant, N.J., and Blatt, M.R. (2013). Arabidopsis Sec1/Munc18 Protein SEC11 Is a Competitive and Dynamic Modulator of SNARE Binding and SYP121-Dependent Vesicle Traffic. Plant Cell 25, 1368-1382.

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Secretory Traffic Vectors

Gateway compatible vector for stable fluorescence co-expression and tagging of proteins in Arabidopsis.

For the use of Dex inducible secretory traffic vector please cite:

Grefen, C., Karnik, R., Larson, E., Lefoulon, C., Wang, Y., Waghmare, S., Zhang, B., Hills, A., and Blatt, M.R. (2015). A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion. Nature Plants 1, 15108.

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FRET Vectors

Binary 2-in-1 FRET vector set for dynamic protein interaction studies.

Binary 2-in-1 Vector for detecting voltage dependent conformation changes by FRET.

For the use of 2-in-1 FRET vectors please cite:

Hecker, A., Wallmeroth, N., Peter, S., Blatt, M.R., Harter, K., and Grefen, C. (2015). Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies. Plant Physiol 168, 776-787.

For the use of the pVd FRET vector please cite:

Grefen, C., Karnik, R., Larson, E., Lefoulon, C., Wang, Y., Waghmare, S., Zhang, B., Hills, A., and Blatt, M.R. (2015). A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion. Nature Plants 1, 15108.

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Vector Conversion

Conversion Vectors for 2-in-1 cloning.

For the use of 2-in-1 Conversion Vectors please cite:

Grefen,C., and Blatt,M.R. (2012) “A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC).” Biotechniques 53,311-14.

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rBiFC Vectors

Binary 2-in-1 vector set enabling ratiometric Bimolecular Fluorescence Complementation (rBiFC).

For the use of rBiFC Vectors please cite:

Grefen,C., and Blatt,M.R. (2012) “A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC).” Biotechniques 53,311-14.

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